Recombinant SARS-CoV-2 NSP3 protein
Size: 50μg
Catalogue Number: PKSV030325-50
Citations, Manuals and MSDS Available upon request.
Abbreviation: NSP3
Target Symptom: nsp3; PL-PRO; Papain-like proteinase; X domain(Macro domain)
Target Species: SARS-CoV-2
Expression Host: E.coli
Fusion Tag: N-His
UNIProt ID: P0DTC2
Accession: QHD43415.1
Background: The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.
Sequence: Glu1024-Gln1198
Purity: > 90 % as determined by reducing SDS-PAGE.
Formulation: Lyopilized from PBS pH7. 4, 0.02%NLS, 1mM EDTA, 4%trehalose, 1% mannitol.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: Please refer to the printed manual for detailed information.
Endotoxin: Please contact us for more information.
Calculated MW: 22.03 kDa
Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Research Use Only