Recombinant Smad2 Monoclonal Antibody
Sizes: 50μL, 100μL
Catalogue Numbers: E-AB-81486-50, E-AB-81486-100
Citations, Manuals and MSDS Available upon request.
Abbreviation: Smad2
Target Synonym: Drosophila; homolog of; MADR2; hMAD-2; HsMAD2; JV18; JV18-1; JV181; MAD; MAD homolog 2; MAD Related Protein 2; Mad-related protein 2; MADH2; MADR2; MGC22139; MGC34440; Mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic; Drosophila; homolog of; 2; Mothers against DPP homolog 2; OTTHUMP00000163489; Sma and Mad related protein 2; Sma- and Mad-related protein 2 MAD; SMAD 2; SMAD family member 2; SMAD; mothers against DPP homolog 2; SMAD2; SMAD2
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Rat, Hamster
Application: WB, IHC-P
Isotype: IgG
Clonality: Rabbit Monoclonal
Clone No.: R03-3F8
UNIProt ID: Q15796
Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Concentration: 300 μg/mL
Immunogen: A synthetic peptide of human Smad2
Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Purification Method: Affinity Purified
Dilution: WB 1:1000-1:5000 IHC 1:20-1:100
Calculated MW: 52kDa
Observed MW: 52kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only