RFC5 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-62196-60, E-AB-62196-120, E-AB-62196-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: RFC5
Target Synonym: RFC5; RFC36
Research Areas: Epigenetics and Nuclear Signaling
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P40937
Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.
Concentration: 1 mg/mL
Immunogen: Recombinant fusion protein of human RFC5
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000
Calculated MW: 36kDa/38kDa
Observed MW: 38kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only