12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK8156-48, ELK8156-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 12HETE
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 5.32 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.