17-OHP (17-Hydroxyprogesterone) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7875-48, ELK7875-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 87.3 pg/mL
Standard: 20000 pg/mL
Detection Range: 312.5-20000 pg/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 2h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 17-OHP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-OHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.