5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit
Sizes: 24T, 48T, 96T
Catalogue Numbers: E-EL-0116-24T, E-EL-0116-48T, E-EL-0116-96T
Citations, Manuals and MSDS Available upon request.
Abbreviation: 5-HETE
Target Synonyms: 5-HETE; 5(S) -HETE; 5S-HETE;
Research Areas: Signal transduction, Metabolism, Cardiovascular, Cardiovascular
Target Species: Universal
Application: ELISA
Usage: This ELISA kit applies to the in vitro quantitative determination of 5-HETE concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, Plasma and other biological fluids
Type: Competitive
Assay Time: 2.5h
Detection Type: Colormetric
Sensitivity: 0.09 ng/mL
Detection Range: 0.16-10 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 5-HETE. During the reaction, Universal 5-HETE in the sample or standard competes with a fixed amount of Universal 5-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 5-HETE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 5-HETE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross Reactivity: This kit recognizes Universal 5-HETE in samples. No significant cross-reactivity or interference between Universal 5-HETE and analogues was observed
Recovery Rate: 80%-120%
Precision: Both intra-CV and inter-CV are < 10%.
Storage: 2-8℃/-20℃, 12 months
Shipping: ice bag