5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8155-48, ELK8155-96, ELK8155-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 5-HETE
Assay Type: Competitive Inhibition
Sensitivity: 3.83 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Enzyme and Kinase
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only