5-Me-THF (5-Methyltetrahydrofolate) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK8059-48, ELK8059-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 5-Me-THFA; 5-Me-H4FA; 5-Me-THF; 5-Me-H4F; Levomefolic Acid; Metafolin; 5-Methyltetrahydrofolic Acid; L-Methylfolate
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.43 ng/mL
Standard: 90 ng/mL
Detection Range: 1.41-90 ng/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Hematology; Reproductive science; Nutrition metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Me-THF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Me-THF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.