8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10848-48, ELK10848-96, ELK10848-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type: Competitive Inhibition
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Tumor immunity; Infection immunity; Endocrinology; Hematology; Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only