8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7861-48, ELK7861-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 26.29 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Metabolic pathway; Tumor immunity; Infection immunity; Endocrinology; Hematology; Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.