AA (Arachidonic Acid) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK11240-48, ELK11240-96, ELK11240-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ARA
Assay Type: Competitive Inhibition
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Signal transduction; Metabolic pathway; Endocrinology; Neuroscience; Hormone metabolism; Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only