AcCoA (Acetyl CoA) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9575-48, ELK9575-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: AcCoA
Species: General
Assay Type: Sandwich
Sensitivity: 0.119 nmol/mL
Standard: 20 nmol/mL
Detection Range: 0.32-20 nmol/mL
Sample Type: Cell Lysate, Tissue Lysate
Assay length: 3.5h
Research Areas: Metabolic pathway; Signal Transduction; Cancer
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AcCoA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AcCoA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AcCoA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AcCoA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.