ACTb (Actin Beta) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK8833-48, ELK8833-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 2h
Research Areas: Signal transduction; Developmental science; Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ACTb protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACTb in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.