ADT (Androsterone) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7926-48, ELK7926-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 144 pg/mL
Standard: 90000 pg/mL
Detection Range: 1406.25-90000 pg/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ADT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ADT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADT in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.