AGE (Advanced Glycation End Product) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK1890-48, ELK1890-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: AGEs; Advanced Glycation End Products
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 38.2 ng/mL
Standard: 8000 ng/mL
Detection Range: 125-8000 ng/mL
Sample Type: Serum, Plasma and other Biological Fluids
Assay length: 2h
Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGE in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.