Avian bHB (Beta-Hydroxybutyric Acid) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK8677-48, ELK8677-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid
Species: Avian
Assay Type: Competitive Inhibition
Sensitivity: 0.48 µg/mL
Standard: 100 µg/mL
Detection Range: 1.57-100 µg/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 2h
Research Areas: Signal transduction; Metabolic pathway; Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Avian Avian bHB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Avian Avian bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Avian Avian bHB in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.