BA (Butyric Acid) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK8174-48, ELK8174-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Propanecarboxylic Acid; Butanoic Acid
Species: General
Assay Type: Competitive Inhibition
Sensitivity: 51.5 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Biological agents
Assay length: 2h
Research Areas: Signal transduction; Metabolic pathway; Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with BA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.