Cattle 17-OHP (17-Hydroxyprogesterone) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8686-48, ELK8686-96, ELK8686-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 17OHP; 17-OH Progesterone; 17-Alpha-Hydroxyprogesterone
Assay Type: Competitive Inhibition
Sensitivity: 88.5 pg/mL
Standard: 20000 pg/mL
Detection Range: 312.5-20000 pg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle 17-OHP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle 17-OHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only