Cattle bEP (Beta-Endorphin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7148-48, ELK7148-96, ELK7148-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Beta EP; b-EP
Assay Type: Competitive Inhibition
Sensitivity: 4.71 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Signal transduction; Endocrinology; Neuroscience; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle bEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle bEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle bEP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only