Cattle CSN2 (Casein Beta) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7989-48, ELK7989-96, ELK7989-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CASb; Beta-casein; CASB; CASB_HUMAN; Casein beta; CSN 2; Csn2; Casein beta S2
Assay Type: Sandwich
Sensitivity: 38 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: milk
Assay Length: 3.5h
Research Areas: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle CSN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle CSN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle CSN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle CSN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02666
Research Use Only