Cattle ECF (Eosinophil Chemotactic Factor) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK5968-48, ELK5968-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Species: Cattle
Assay Type: Sandwich
Sensitivity: 21.8 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: Serum, Plasma and other Biological Fluids.
Assay length: 3.5h
Research Areas: Cytokine; Infection immunity; Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle ECF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle ECF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle ECF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle ECF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.