Cattle GC (Glucagon) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9240-48, ELK9240-96, ELK9240-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone
Assay Type: Competitive Inhibition
Sensitivity: 7.39 pg/mL
Standard: 1500 pg/mL
Detection Range: 23.44-1500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle GC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01272
Research Use Only