Cattle PPAR-γ (Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK6488-48, ELK6488-96, ELK6488-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma
Assay Type: Sandwich
Sensitivity: 0.132 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Developmental science; Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O18971
Research Use Only