Cattle SAA (Serum Amyloid A) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10656-48, ELK10656-96, ELK10656-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein
Assay Type: Sandwich
Sensitivity: 0.273 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Infection immunity; Kidney biomarker; Hepatology; Urology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle SAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle SAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle SAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle SAA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P35541
Research Use Only