Cattle T3 (Triiodothyronine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0911-48, ELK0911-96, ELK0911-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin
Assay Type: Competitive Inhibition
Sensitivity: 0.072 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Autoimmunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle T3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle T3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle T3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only