Cattle TRH (Thyrotropin Releasing Hormone) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9239-48, ELK9239-96, ELK9239-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
Assay Type: Competitive Inhibition
Sensitivity: 2.75 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Neuroscience; Hepatology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only