Cattle TSP-1 (Thrombospondin-1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10705-48, ELK10705-96, ELK10705-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type: Sandwich
Sensitivity: 0.43 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample Type: Serum and plasma
Assay Length: 3.5h
Research Areas: Infection immunity; Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q28178
Research Use Only