Chicken 8-iso-PGF2α (8-isoprostane) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9298-48, ELK9298-96, ELK9298-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 8-isoprostane; 8-iso-PGF2α
Assay Type: Competitive Inhibition
Sensitivity: 9.511 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.625-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Endocrinology; Pulmonology; Hepatology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken 8-iso-PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken 8-iso-PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken 8-iso-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only