Chicken GSH (Glutathione) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10146-48, ELK10146-96, ELK10146-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: L-Reduced Glutathione
Assay Type: Competitive Inhibition
Sensitivity: 0.42 µg/mL
Standard: 100 µg/mL
Detection Range: 1.56-100 µg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2.5h
Research Areas: Metabolic pathway
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GSH in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only