Chicken VEGFA (Vascular Endothelial Growth Factor A) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2037-48, ELK2037-96, ELK2037-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
Assay Type: Sandwich
Sensitivity: 6.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Areas: Cytokine; Apoptosis; Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken VEGFA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken VEGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken VEGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken VEGFA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P67964
Research Use Only