CLIA Kit for Coagulation Factor VIII (F8)
Size: 96T
Catalogue Number: USCB878Hu-96
Citations, Manuals and MSDS Available upon request.
Target: Coagulation Factor VIII (F8)
Alternative Names: FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
Target Species: Human
Detection range: 13.7-10,000pg/mL
Sensitivity: 4.9pg/mL
Uniprot: P00741
Featured Series Function: Detects protein (regular version)
Recommended/Predicted Sample Types: Plasma
Assay Time: 2h, 40min
Method: Chemiluminescence
Specificity: Reactive with Human F8 / Coagulation Factor VIII
Detection principle: Double-antibody Sandwich
Sample Size: 100uL
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8).
No significant cross-reactivity or interference between Coagulation Factor VIII (F8) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Coagulation Factor VIII (F8) level in the sample or standard.;
Research Area: Metabolic pathway; Hematology;
Research Use Only