CNCbl (Cyanocobalamin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7871-48, ELK7871-96, ELK7871-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide
Assay Type: Competitive Inhibition
Sensitivity: 49.1 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Nutrition metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CNCbl. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CNCbl. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CNCbl in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only