DDAVP (1-Desamino 8D Arginine Vasopressin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7864-48, ELK7864-96, ELK7864-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: d-DAVP; Stimate; Minirin; Desmopressin
Assay Type: Competitive Inhibition
Sensitivity: 38.4 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DDAVP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DDAVP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DDAVP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only