Dog GHRL (Ghrelin) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK9731-48, ELK9731-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: MTLRP, Growth Hormone-Releasing Peptide
Species: Dog
Assay Type: Competitive Inhibition
Sensitivity: 51.3 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Signal transduction, Metabolic pathway, Endocrinology, Gastroenterology, Hormone metabolism,
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog GHRL protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog GHRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog GHRL in the samples is then determined by comparing the OD of the samples to the standard curve.
UNIProt ID: Q9BEF8
Research Use Only