Dog HbA1c (Glycated Hemoglobin A1c) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9768-48, ELK9768-96, ELK9768-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
Assay Type: Competitive Inhibition
Sensitivity: 4.07 µg/mL
Standard: 1000 µg/mL
Detection Range: 15.63-1000 µg/mL
Sample Type: plasma and erythrocyte lysates
Assay Length: 2h
Research Areas: Diagnosis of diabetes mellitus
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only