Dog SDMA (Symmetric dimethylarginine) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8834-48, ELK8834-96, ELK8834-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Symmetric dimethylarginine
Assay Type: Competitive Inhibition
Sensitivity: 0.051 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Signal transduction; Developmental science; Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog SDMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SDMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SDMA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only