EasyStep Human MCP1 (Monocyte Chemotactic Protein 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK071ES-48, ELK071ES-96, ELK071ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE
Assay Type: Sandwich
Sensitivity: 0.57 pg/mL
Standard: 500 pg/mL
Detection Range: 7.81-500 pg/mL
Sample Type: Serum, plasma, cell culture supernatants
Assay Length: 1.5h
Research Areas: Cytokine; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP1, and the Human MCP1 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MCP1. After TMB substrate solution is added, only those wells that contain Human MCP1 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only