EasyStep Human MMP9 (Matrix Metalloproteinase 9) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK041ES-48, ELK041ES-96, ELK041ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
Assay Type: Sandwich
Sensitivity: 1.25 ng/mL
Standard: 80 ng/mL
Detection Range: 1.25-80 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Enzyme and Kinase; Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P14780
Research Use Only