EasyStep Human sTFR (Soluble Transferrin Receptor) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK092ES-48, ELK092ES-96, ELK092ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1
Assay Type: Sandwich
Sensitivity: 17.55 ng/mL
Standard: 5000 ng/mL
Detection Range: 78.13-5000 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Signal transduction; CD and Adhesion molecule; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTFR, and the Human sTFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human sTFR. After TMB substrate solution is added, only those wells that contain Human sTFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only