EasyStep Human TF (Tissue Factor) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK073ES-48, ELK073ES-96, ELK073ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
Assay Type: Sandwich
Sensitivity: 0.12 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Hematology; Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TF, and the Human TF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TF. After TMB substrate solution is added, only those wells that contain Human TF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only