EasyStep Human TRF (Transferrin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK066ES-48, ELK066ES-96, ELK066ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
Assay Type: Sandwich
Sensitivity: 11.9 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Tumor immunity; Infection immunity; Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TRF. After TMB substrate solution is added, only those wells that contain Human TRF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only