EasyStep Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK088ES-48, ELK088ES-96, ELK088ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
Assay Type: Sandwich
Sensitivity: 32.1 ng/mL
Standard: 10 µg/mL
Detection Range: 0.16-10 µg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: CD and Adhesion molecule; Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin, and the Human VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VE-Cadherin. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only