EasyStep Mouse APOB (Apolipoprotein B) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK094ES-48, ELK094ES-96, ELK094ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Apo-B; FLDB; Apo B-100; Apo B-48
Assay Type: Sandwich
Sensitivity: 43.2 ng/mL
Standard: 12 μg/mL
Detection Range: 0.19-12 μg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOB, and the Mouse APOB standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse APOB. After TMB substrate solution is added, only those wells that contain Mouse APOB and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOB in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only