EasyStep Mouse E2 (Estradiol) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK021ES-48, ELK021ES-96, ELK021ES-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type: Competitive Inhibition
Sensitivity: 25 pg/mL
Standard: 1600 pg/mL
Detection Range: 25-1600 pg/mL
Sample Type: Serum
Assay Length: 1.5h
Research Areas: Endocrinology; Reproductive science; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2 antigen, and the Mouse E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only