GA (Gibberellic Acid) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK7853-48, ELK7853-96, ELK7853-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: GA3; Gibberellin A3; Gibberellins
Assay Type: Competitive Inhibition
Sensitivity: 52.8 ng/mL
Standard: 10000 ng/mL
Detection Range: 156.25-10000 ng/mL
Sample Type: tissue or cell culture supernates
Assay Length: 2h
Research Areas: Nutrition metabolism; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with GA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only