Human aCTx (Alpha-Crosslaps) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4853-48, ELK4853-96, ELK4853-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction
Assay Type: Competitive Inhibition
Sensitivity: 25.7 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, other biological fluids
Assay Length: 2h
Research Areas: Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only