Human Anti-IgA (Anti-Immunoglobulin A Antibody) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8046-48, ELK8046-96, ELK8046-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: anti-IgA
Assay Type: Sandwich
Sensitivity: 2.38 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Areas: Infection immunity; Immune molecule; Hematology; Pulmonology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only