Human Anti-MBP (Anti-Myelin Basic Protein Antibody) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8048-48, ELK8048-96, ELK8048-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Myelin A1 protein; Myelin membrane encephalitogenic protein
Assay Type: Competitive Inhibition
Sensitivity: 1.27 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2.5h
Research Areas: Infection immunity; Neuroscience; Autoimmunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-MBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-MBP in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only