Human ASS1 (Argininosuccinate Synthetase 1) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK3621-48, ELK3621-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CTLN1; Citrulline--aspartate ligase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.56 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, Plasma, Tissue Homogenates and other Biological Fluids
Assay length: 3.5h
Research Areas: Enzyme and Kinase; Metabolic pathway; Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASS1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P00966
Research Use Only.