Human ATG12 (Autophagy Related Protein 12) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7517-48, ELK7517-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: APG12; APG12L; HAPG12; Ubiquitin-like protein ATG12
Species: Human
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue Homogenates, Cell Lysates and other Biological Fluids
Assay length: 3.5h
Research Areas: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATG12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATG12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATG12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATG12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O94817
Research Use Only.